Journal: BioMed Research International
Article Title: L Antigen Family Member 3 Serves as a Prognostic Biomarker for the Clinical Outcome and Immune Infiltration in Skin Cutaneous Melanoma
doi: 10.1155/2021/6648182
Figure Lengend Snippet: Expression analysis of LAGE3. (a) The mRNA expression of LAGE3 between the tumor and normal tissues (ACC, BLCA, BRCA, CESC, CHOL, COAD, ESCA, GBM, HNSC, KICH, KIRC, KIRP, LAML, LGG, LIHC, LUAD, LUSC, OV, PAAD, PRAD, READ, SKCM, STAD, TGCT, THCA, UCEC, and UCS) was assessed using tissues from TCGA and GTEx. (b–e) LAGE3 mRNA expression levels by (b) GSE98394, (c) GSE3189, (d) TCGA combined with GTEx, and (e) GSE46517. ANOVA was used for comparisons between the different groups. (f, g) LAGE3 mRNA expression levels and protein expression levels in normal human epidermal melanocytes (HEMa-LP) and melanoma cell lines (A375, SK-MEL-2, M21, and MEL-RM). The numbers were the expression of LAGE3 protein relative to GAPDH, and the normal epidermal cell expression was set as 1. (h) Representative immunohistochemistry images and detailed information about LAGE3 in melanoma tissues and normal tissues using HPA. ACC: adrenocortical carcinoma; BLCA: bladder urothelial carcinoma; BRCA: breast invasive carcinoma; CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma; CHOL: cholangiocarcinoma; COAD: colon adenocarcinoma; ESCA: esophageal carcinoma; GBM: glioblastoma multiforme; HNSC: head and neck squamous cell carcinoma; KICH: kidney chromophobe; KIRC: kidney renal clear cell carcinoma; KIRP: kidney renal papillary cell carcinoma; LAML: acute myeloid leukemia; LGG: brain lower grade glioma; LIHC: liver hepatocellular carcinoma; LUAD: lung adenocarcinoma; LUSC: lung squamous cell carcinoma; OV: ovarian serous cystadenocarcinoma; PAAD: pancreatic adenocarcinoma; PRAD: prostate adenocarcinoma; READ: rectum adenocarcinoma; SKCM: skin cutaneous melanoma; STAD: stomach adenocarcinoma; TGCT: testicular germ cell tumors; THCA: thyroid carcinoma; UCEC: uterine corpus endometrial carcinoma; UCS: uterine carcinosarcoma.
Article Snippet: Normal human epidermal melanocytes, adult, the lightly pigmented donor (HEMa-LP) were obtained from Invitrogen and cultured in Medium 254 (Cascade Biologics), supplemented with human melanocyte growth supplement (Cascade Biologics).
Techniques: Expressing, Immunohistochemistry